LabSolutions LCMS has been listed in Japan recently with SHIMADZU LC/MS/MS short chain fatty acid analysis.
If we want to analyze the short chain fatty acids produced by intestinal microflora by LC/MS, is there a reasonable measurement platform for derivative steps?
Please use the LCMS-8060 and the short chain fatty acid analysis package.
Characteristics of this method package
Analysis objects -- short chain fatty acids and organic acids
It is known that the short chain fatty acids produced by intestinal bacteria include acetic acid, propionic acid, and butyric acid, which are reported to be associated with obesity and diabetes and other living habits.
Usually, short chain fatty acids have high volatility and high hydrophilicity, so it is difficult to carry out LCMS analysis in normal reverse phase system. Therefore, the method of 3- package will 4-dinitrophenylhydrazine (3-NPH) short chain fatty acid derivatives (C2 to C5) as the analysis object, by setting the MRM ion pair, simultaneous analysis of organic acids and central metabolic pathways (22 components).
The setting of the MRM transition
In the setting of MRM transition, the characteristic product ions set 3- picrylhydrazyl derivatives, can improve the selectivity. In addition, some organic acids containing carbonyl ketone derivatives from 3-, 4-dinitrophenylhydrazine derivatives and carboxylic acid and carbonyl reaction so as to the object of MRM ion.
Preconditioning including derivatization
The package instructions covering the pretreatment program including 3- 4-dinitrophenylhydrazine derivatization steps, in accordance with the steps, can carry out the derivatization ~ analysis and analytical work immediately after the introduction of.
Contact: ChunMing Guo
Add: 武汉市汉阳大道恒大绿洲27栋3单元 27 3 units of Hengda oasis, Hanyang Avenue, Wuhan